Chester stretched their unbeaten run to 12 games with an excellent point against one of their closest rivals for promotion.
The result meant that they were overtaken at the top by Yeovil but manager Mark Wright was happy with the outcome after Doncaster had created the better chances.
The home side forced the pace for most of the match but found the solid Chester defence a tough nut to crack.
Centre backs Carl Ruffer, Phil Bolland and Scott Guyett were dominant in defence and although Doncaster did manage to carve out openings their finishing was poor.
Andy Watson hit a low 20-yard effort just wide in the 26th minute before Wayne Brown saved an angled effort from Justin Jackson five minutes later.
It was the first save of the game from either keeper as both sides adopted a cagey approach and defended in numbers when the need arose.
Ryan Sugden fired wide from Chester's only first-half scoring opportunity but play was more even after the interval, although Doncaster continued to dictate the pace.
Paul Barnes missed a good chance for Doncaster straight after the interval when his first-time shot came back off the keeper's legs, following a mistake by Bolland.
Kevin McIntyre produced a couple of last-ditch tackles to deny the home side before Brown produced a brilliant save to keep out a glancing header from Barnes in the 54th minute.
Michael Twiss had a chance for Chester in the 70th minute but failed to connect properly with the ball and then Sugden had a low effort well saved by Andy Warrington.
The best chance fell to Doncaster nine minutes from time when Barnes picked out Mark Albrighton unmarked in the middle only to see him place his close-range header wide of the mark