Darlington had to fight to the very end to ensure that they secured the three points after coming from behind to defeat Doncaster 2-1 at the Reynolds Arena.
Newly promoted Rovers battled all the way and were disappointed at the end that they had failed to get anything from the game.
Rovers are adjusting to life back in the Third Division following last season's successful Conference campaign.
But Darlington are proving a hard nut to crack in their new stadium and this was their third straight win. However, they had to thank second half substitute Neil Wainwright, who came off the bench to score both of Darlington's goals.
The first half was entertaining, although neither side had managed to find the back of the net. Darlington went closest when striker Barry Conlon headed against the bar in the 34th minute.
Doncaster almost grabbed the lead early in the second half when midfielder Michael McIndoe went close with a curling drive and a minute later captain Steve Foster fired straight at Darlington keeper Andy Collett.
The visitors did take the lead in the 52nd minute thanks to a penalty from midfielder Jamie Paterson after Paul Green had been brought down by Gary Pearson.
A double substitution from the home side saw Wainwright and Ian Clark into the Arena and within a minute Wainwright grabbed the equaliser when he converted a Matthew Clarke cross from close range.
Wainwright put the home side into the lead in the 67th minute when he converted from 12 yards out after a Clark cross was only half cleared.
Wainwright had a chance to kill the game off and grab a hat-trick in the process, but in the 70th minute he somehow fired into the side netting.
Rovers launched a late rally and McIndoe went close with a 77th minute free kick and then Barnes had a goal bound effort deflected away.
Green fired wide in the 86th minute and in the dying seconds Foster was booked for deliberate handball to end a miserable day for the South Yorkshire outfit.
After the game Doncaster boss David Penney felt his side failed to perform.
He said: "That is probably the worst we have played since we came back up and we are still adjusting to life in the Third Division but we lacked fluidity." Darlington manager Mick Tait said: "The fans are getting behind us and with the vastness of the new stadium the sound really echoes around. We knew we had to change things around and they paid off instantly."